脂肪组织特异性PU.1敲除小鼠的鉴定及其活体脂肪和肌肉含量分析

doi:10.11843/j.issn.0366-6964.2014.02.009

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (2): 225-232.doi: 10.11843/j.issn.0366-6964.2014.02.009

脂肪组织特异性PU.1敲除小鼠的鉴定及其活体脂肪和肌肉含量分析

庞卫军^1,2*，卫宁¹，王禹¹，熊燕¹，童强²，杨公社¹

（1.西北农林科技大学动物脂肪沉积与肌肉发育实验室，杨凌 712100； 2.贝勒医学院，美国休斯顿 77030）

收稿日期:2013-06-28 出版日期:2014-02-23 发布日期:2014-02-23
通讯作者: 庞卫军，E-mail：pwj1226@nwsuaf.edu.cn
作者简介:庞卫军（1972-），男，陕西汉中人，副教授，博士，主要从事动物遗传育种研究
基金资助:
国家“973”计划子项目（2012CB124705）；国家自然科学基金（30600437）；西北农林科技大学基本科研业务项目（QN2009021）；国家生猪产业技术体系（CARS-36）

Identification of Adipose-specific PU.1 Knockout Mice and Analysis of Their Live Body Fat and Lean Contents

PANG Wei-jun^1，2*，WEI Ning¹，WANG Yu¹，XIONG Yan¹，TONG Qiang²，YANG Gong-she¹

(1.Laboratory of Animal Fat Deposition and Muscle Development，Northwest A & F University，Yangling 712100，China； 2.Baylor College of Medicine，Houston 77030，USA)

Received:2013-06-28 Online:2014-02-23 Published:2014-02-23

摘要/Abstract

摘要：

本研究运用基因工程和同源重组等技术，产生了脂肪组织特异性PU.1敲除小鼠(aP2-cre-PU.1^fl/fl)，并用PCR技术进行基因型判定。Western blot结果表明，与PU.1^fl/fl小鼠相比，aP2-cre-PU.1^fl/fl小鼠脂肪组织PU.1蛋白表达显著降低，达到敲除水平。在此基础上，对1～6月龄的PU.1^fl/fl和aP2-cre-PU.1^fl/fl小鼠体重和活体成分进行了分析。研究发现，aP2-cre-PU.1^fl/fl小鼠在出生后第5和第6月龄体重显著高于PU.1^fl/fl小鼠(P<0.05)；6月龄时，肌肉量无显著差异，但aP2-cre-PU.1^fl/fl小鼠脂肪量明显高于PU.1^fl/fl小鼠(P<0.05)。结果提示，aP2-cre-PU.1^fl/fl小鼠脂肪重量的增加是其体重增加的主要原因。脂肪组织特异性PU.1敲除小鼠的生产为深入研究PU.1基因在体调控脂肪生成的功能奠定了基础，也为探索PU.1基因控制家畜体脂沉积提供了理论依据。

Abstract:

aP2-cre-PU.1^fl/fl mice were generated and identified using gene engineering，homologous recombination，PCR and Western blotting technologies in this study.The results showed that，compared with PU.1^fl/fl mice,PU.1 protein was down-regulated to the level of that of aP2-cre-PU.1^fl/fl.The body weight and body composition were analyzed in PU.1^fl/fl and aP2-cre-PU.1^fl/fl mice with 1-6 month old.Moreover，the body weight of aP2-cre-PU.1^fl/fl mice was significantly higher than that of PU.1^fl/fl mice at month 5 and month 6 (P<0.05).There was no significant difference in muscle weight but fat weight was significantly higher in aP2-cre-PU.1f^l/fl mice than that in PU.1^fl/fl mice (P<0.05).The results suggest that increase of adipose tissue of aP2-cre-PU.1^fl/fl mice results in the increase of their body weight.Therefore，aP2-cre-PU.1^fl/fl mice is the better animal model to explore PU.1 physiological functions in adipose tissue，which provide the theorical basis for exploring the effect of PU.1 gene on fat deposition in animals.

中图分类号:

Q343.15

庞卫军，卫宁，王禹，熊燕，童强，杨公社. 脂肪组织特异性PU.1敲除小鼠的鉴定及其活体脂肪和肌肉含量分析[J]. 畜牧兽医学报, 2014, 45(2): 225-232.

PANG Wei-jun，WEI Ning，WANG Yu，XIONG Yan，TONG Qiang，YANG Gong-she. Identification of Adipose-specific PU.1 Knockout Mice and Analysis of Their Live Body Fat and Lean Contents[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 225-232.

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脂肪组织特异性PU.1敲除小鼠的鉴定及其活体脂肪和肌肉含量分析

Identification of Adipose-specific PU.1 Knockout Mice and Analysis of Their Live Body Fat and Lean Contents

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